Fast, Accurate and Low Cost Method of Quantification of Multiple Nucleic Acid Targets In Complex Mixtures
Currently there is no technology that allows for a fast, accurate and low cost analysis of multiple nucleic acid targets (up to 1000) in biological samples. Researchers are dependent on tests that are time consuming, expensive or have severe limitations. qPCR the “golden standard” for quantification of nucleic acid implementation in some biomedical applications (such as tumor profiling) is limited due to an insufficient level of multiplexing (up to 5 targets in the same tube) and microarrays, which can analyze thousands of targets in one sample, take a very long time and are expensive to run.
This invention combines the powers of two widely used approaches from molecular biology (qPCR) and nanotechnology to fill the gap between low- and highthroughput techniques to profile gene expression and other genetic variations.
This technique can be used to quantify multiple nucleic acids targets such as gene expression, copy number variations, protein isoforms, gene translocation, microRNA and other genetic variations at up to the single-cell level. In addition, it is very sensitive, accurate, fast and easy to use thus decreasing costs and expanding potential biological and biomedical applications such as molecular diagnostic assays, pathogen detection and identification or gene expression quantification in cancer diagnostics and tumor profiling.
Single-cell molecular profiling
Has been successfully applied to test multiple targets in various human samples.